Journal: International Journal of Molecular Sciences

Article Title: P38α-MAPK Signaling Inhibition Attenuates Soleus Atrophy during Early Stages of Muscle Unloading

doi: 10.3390/ijms21082756

Figure Lengend Snippet: Evaluation of phospho-p38 content in soleus muscles of non-treated control (C), 3 days of unloading/hindlimb suspension (HS), and 3 days HS with VX-745 inhibitor (HSVX) rats by Western blotting. Values are normalized to the levels of total protein and total-p38 expression in each sample. n = 8. * indicates a significant difference from the control, p < 0.05; # indicates a significant difference from the HS, p < 0.05.

Article Snippet: Rats were randomly assigned to one of the three groups with eight animals per group: non-treated control (C), or three days of hindlimb suspension/unloading with (HSVX) or without (HS) p38α inhibitor VX-745 (10 mg/kg/day orally with small amount of food soaked in VX-745 solution; #A8686, APExBio, Houston, TX, USA).

Techniques: Western Blot, Expressing

Journal: bioRxiv

Article Title: Opposing roles of p38α-mediated phosphorylation and arginine methylation in driving TDP-43 proteinopathy

doi: 10.1101/2021.08.04.455154

Figure Lengend Snippet: A) Schematic of domain architecture of TDP-43 and location of ALS-linked mutations and phosphorylation sites (P) detected after p38α MAPK treatment in vitro . B) Western blot of total and phosphorylated TDP-43 M337V in RIPA and urea fractions of SH-SY5Y cells with siRNA-induced p38α knockdown. GAPDH was used as a loading control. C) Quantification of urea/RIPA ratio of total TDP-43 normalized to levels in scrambled siRNA (si-scr). D) Quantification of pTDP-43/total TDP-43 ratio in urea fraction normalized to levels in si-scr (mean band signal ± SD, two-way ANOVA with Sidak’s multiple comparison test, n = 3). E) Western blot of total and pTDP-43 M337V in RIPA and urea fractions of SH-SY5Y cells with pharmacological p38α inhibition with compound 1. GAPDH was used as a loading control. Quantification of urea/RIPA ratio of total TDP-43 at time points 24h (F) and 48h (G) post-transfection, and pTDP-43/total TDP-43 ratio in urea fraction at time point 48h post-transfection (H) normalized to levels in DMSO-treated cells (mean band signal ± SD, one-way ANOVA with Dunnett’s multiple comparison test, n = 3). I) Quantification of LDH activity in conditioned medium normalized to levels in DMSO-treated TDP-43 WT -transfected NSC-34 cells. (one-way ANOVA with Dunnett’s multiple comparison test, n = 4 with 6 replicates in each). ∗ p < 0.05, ∗∗ p < 0.01 ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. J) Hazard ratios of primary neurons expressing mApple and TDP-43 M337V -EGFP treated with p38α inhibitor VX-745 at 0.3, 1, 3, and 9 µM compared with DMSO control (reference, set at 1.0) were 0.6296, 0.6378, 0.4596, and 0.6869 respectively. Reduction of hazard ratio was most significant at 3 µM (Cox proportional hazard, p<0.01). See also .

Article Snippet: Neurons were treated with p38 α inhibitor, VX-745 (synthesized by AstraZeneca), at 0.3, 1, 3, or 9 µM, and imaged daily for 7 days on a custom-built highthroughput robotic microscopy system ( ; ).

Techniques: In Vitro, Western Blot, Inhibition, Transfection, Activity Assay, Expressing

Journal: The EMBO Journal

Article Title: Immediate early splicing controls translation in activated T-cells and is mediated by hnRNPC2 phosphorylation

doi: 10.1038/s44318-025-00374-8

Figure Lengend Snippet: Reagents and tools table

Article Snippet: VX-745 (p38 inhibitor) , Biomol , Cay18075-1.

Techniques: Recombinant, Construct, Sequencing, Reverse Transcription, SYBR Green Assay, Marker, Western Blot, Software, Electroporation, Blocking Assay, Imaging, Mass Spectrometry